Optogenetic feedback control of neural activity.

TitleOptogenetic feedback control of neural activity.
Publication TypeJournal Article
Year of Publication2015
AuthorsNewman, JP, Fong, M-fai, Millard, DC, Whitmire, CJ, Stanley, GB, Potter, SM
JournalElife
Volume4
Paginatione07192
Date Published2015
ISSN2050-084X
KeywordsAction Potentials, Cytological Techniques, Feedback, Humans, Neurons, Optogenetics
Abstract

Optogenetic techniques enable precise excitation and inhibition of firing in specified neuronal populations and artifact-free recording of firing activity. Several studies have suggested that optical stimulation provides the precision and dynamic range requisite for closed-loop neuronal control, but no approach yet permits feedback control of neuronal firing. Here we present the 'optoclamp', a feedback control technology that provides continuous, real-time adjustments of bidirectional optical stimulation in order to lock spiking activity at specified targets over timescales ranging from seconds to days. We demonstrate how this system can be used to decouple neuronal firing levels from ongoing changes in network excitability due to multi-hour periods of glutamatergic or GABAergic neurotransmission blockade in vitro as well as impinging vibrissal sensory drive in vivo. This technology enables continuous, precise optical control of firing in neuronal populations in order to disentangle causally related variables of circuit activation in a physiologically and ethologically relevant manner.

DOI10.7554/eLife.07192
Alternate JournalElife
PubMed ID26140329
PubMed Central IDPMC4490717
Grant List1R01NS079757-01 / NS / NINDS NIH HHS / United States
1T90DA032466 / DA / NIDA NIH HHS / United States
2R01NS048285 / NS / NINDS NIH HHS / United States
F31 NS089412 / NS / NINDS NIH HHS / United States
F31NS089412 / NS / NINDS NIH HHS / United States
R01NS085447 / NS / NINDS NIH HHS / United States
T90 DA032466 / DA / NIDA NIH HHS / United States

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