%0 Journal Article %J Nature Methods %D 2014 %T Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy %A Prevedel, Robert %A Yoon, Young-Gyu %A Hoffmann, Maximilian %A Pak, Nikita %A Wetzstein, Gordon %A Kato, Saul %A Schrödel, Tina %A Raskar, Ramesh %A Zimmer, Manuel %A Edward S Boyden %A Vaziri, Alipasha %K Imaging %K Neuroscience %X

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

%B Nature Methods %V 11 %P 727 - 730 %8 05/18/2014 %G eng %U http://www.nature.com/doifinder/10.1038/nmeth.2964 %N 7 %! Nat Meth %R 10.1038/nmeth.2964