Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy

TitleSimultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
Publication TypeJournal Article
Year of Publication2014
AuthorsPrevedel, R, Yoon, Y-G, Hoffmann, M, Pak, N, Wetzstein, G, Kato, S, Schrödel, T, Raskar, R, Zimmer, M, Boyden, ES, Vaziri, A
JournalNature Methods
Volume11
Issue7
Pagination727 - 730
Date Published05/18/2014
ISSN1548-7091
KeywordsImaging, Neuroscience
Abstract

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
URLhttp://www.nature.com/doifinder/10.1038/nmeth.2964
DOI10.1038/nmeth.2964
Short TitleNat Meth

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